<i>Bacillus thuringiensis</i>subsp.<i>israelensis</i>producing endochitinase ChiA74Δsp inclusions and its improved activity against<i>Aedes aegypti</i>

Abstract

We report for the first time the engineering of Bti to produce spore-parasporal body-ChiA74∆sp inclusions in the same sporangium, which are released together following autolysis. Our work lays a foundation for engineering Bti to produce more efficacious combinations of Cry4Aa, Cry4Ba, Cry11Aa, Cyt1Aa and chitinase inclusions.