The two intervening sequences of human beta‐ and gamma‐globin pre‐mRNAs are excised in a preferred temporal order in vitro.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

John David Dignam, Russell M. Lebovitz, R G Roeder · Nucleic Acids Research · 1983 · 11.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +17

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

Haim Aviv, Philip Leder · Proceedings of the National Academy of Sciences · 1972 · 6.9K citations

Life Sciences, Biochemistry, Molecular Biology +10

Efficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +11

Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids

Arnold Berk, Phillip A. Sharp · Cell · 1977 · 3K citations

Life Sciences, Molecular Biology, Molecular Genetics +14

Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates

Robert F. Weaver, Charles Weissmann · Nucleic Acids Research · 1979 · 1.3K citations · Full text

Life Sciences, Genetics, Molecular Biology +5