Role of carbohydrate in human chorionic gonadotropin. Effect of deglycosylation on the subunit interaction and on its in vitro and in vivo biological properties.

Abstract

I The abbreviations used are: hCG, human chorionic gonadotropin or human choriogonadotropin; hCG-da and hCG-d,8, deglycosylated a-and ,8-subunits of hCG; hCG-da+d,8, reconstituted deglycosylated hCG; hCG-da+,8 and hCG-a+d,8, reconstituted mixed hybrids ofhCG; TFMS, trifluoromethane sulfonic acid; PK-cAMP.protein kinasebound cAMP, 2 Portion of this paper (including "Materials and Methods," part of "Results," Figs.1234567, and Tables I-III) are presented in miniprint at the end of this paper.Miniprint is easily read with the aid of a standard magnifying glass.