Journal of Clinical Microbiology · 1997 · 73 citations · 17 references
Veterinary MedicineMicrobiologyVeterinary MicrobiologyDiagnostic MicrobiologyMedical MicrobiologyMolecular DiagnosticsGastrointestinal Pathogen DiagnosticsInfectious Disease Molecular TestingPathogen DetectionRodent Infectious DiseasesRodent ParasitologyVeterinary Diagnostic MicrobiologyFecal PelletsMolecular Pathogen DetectionPcr-based DetectionPolymerase Chain Reaction (Pcr) AssaysPolymerase Chain Reaction (Pcr) DiagnosisPolymerase Chain Reaction (Pcr) DiagnosticsPolymerase Chain Reaction (Pcr)-based DiagnosisTransgenic Mice
A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.
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