THE EFFECT OF HEXOSES AND PENTOSES ON THE FORMATION IN VITRO OF PHOSPHOLIPID BY BRAIN TISSUE AS MEASURED WITH RADIOACTIVE PHOSPHORUS

Abstract

PHOSPHOLIPIDFORMATION BY BRAIN inorganic radiophosphorus initially present in the bath recovered as radiophospholipid per gm. of wet tissue.A striking increase in the recovery of radiophospholipid was observed when glucose, fructose, ma,nnose, and galactose were added to the Ringer's medium containing brain slices.When pentoses were added, no such effect was found.The amounts of radiophospholipid recovered in the presence of glucose, mannose, and galactose were approximately equal.In the presence of these hexoses, the recovery was about 5 times as great as that in the TABLE I Effect of Hexoses and Pentoses on Recovery of Radiophospholipid in Brain Slices and Brain Homogenates All values are expressed as per cent of the added Pa2 recovered as radiophospholipid per gm. of wet tissue.-Tissue preparation Slices Slices Homogenate Period of ncubation hrs.