Computer Simulation Studies with Yeast Hexokinase and Additional Evidence for the Random Bi Bi Mechanism

Abstract

The mechanism of action of yeast hexokinase was studied by computer simulation of the steady state random Bi Bi mechanism. The initial rate, product inhibition, and competitive inhibition studies were found to be consistent with the enzymic mechanism being steady state random but approximated by the rapid equilibrium assumption. Results of studies on both the forward and reverse reaction are presented and much of the seemingly conflicting data in the literature regarding the mechanism appears to be resolved.