Hypersensitivity reactions to acetylsalicylic acid. I. Detection of antibodies in human sera using acetylsalicylic acid attached to proteins through the carboxyl group.

Abstract

Aspirin–protein conjugates (aspirin coupled to rabbit anti-human `O' antibody and to anti-bacteriophage-Fab') were prepared by a method which links aspirin through its carboxyl group. The protein conjugates were used to raise specific aspirin antibodies in guinea-pigs. Such antibodies helped to characterize the other preparations which were subsequently used to test suspected hypersensitive sera for aspirin antibodies. Although an antibody of aspirin specificity was detected it could not be related to the clinical hypersensitivity state.