Virology · 1974 · 23 citations · 0 references
Veterinary MedicineInfectious Disease ControlInfectious DiseasesInfection PreventionInfectious Disease EpidemiologyMedical MicrobiologyBacterial InfectionsDisease EpidemiologyInfectious Disease DetectionInfectious Disease PreventionInfectious Disease Risk FactorsInfectious Disease SurveillanceEpidemiology Of Bacterial InfectionsEpidemiology Of Parasitic InfectionsInfectious Disease Case ManagementInfectious Disease FindingsInfectious Disease Management
The complete nucleotide sequence of the gene 6 from the porcine group (Gp) C rotavirus strain Cowden was determined from gene 6-specific clones selected from a cDNA library and from viral transcript RNA. The gene is 1348 nucleotides in length with a potential initiation codon beginning at nucleotide 25 and a stop codon at nucleotide 1231. The deduced protein contains 402 amino acids. Comparison of the gene 6 from this Gp C strain with sequences in the GenBank data base indicated that this gene shared homology with gene 7 of Gp A rotavirus strain SA-11 (22.9%) and gene 9 of Gp A rotavirus strain UK (22.6%), both of which encode the NS34 protein. In vitro translation products produced from transcripts generated from a gene 6 clone containing the entire open reading frame were not immunoprecipitated with either hyperimmune serum specific for the Gp C Cowden strain or a monoclonal antibody directed against the group antigen (VP6) of the Cowden strain. However, products generated from a full-length gene 5 clone of the Cowden strain were immunoprecipitated by each of these antibodies. These data suggest that in contrast to the Gp A viruses in which the gene 6 encodes the major inner capsid protein VP6, the gene 6 of the Cowden Gp C strain encodes a nonstructural protein corresponding to the NS34 of Gp A rotaviruses.