Reaggregation of Insect Cells as Studied by a New Method of Tissue and Organ Culture

Abstract

Cells of the dissociated pupal fat of saturniid moths reaggregated when maintained in vitro in a new type of chamber that permits the culture to "respire" through a film of polyethylene. The fat-body cells are mutually cohesive, but depend on ameboid hemocytes to bring them together. The hemocytes are also capable of causing the reaggregation of particles of diethylaminoethyl-Sephadex.