Induction of synchronous growth in the yeast phase of Wangiella dermatitidis

Abstract

Synchronous yeast-phase cultures of Wangiella dermatitidis were induced by starvation, heat shock, and inhibition of deoxyribonucleic acid synthesis by hydroxyurea. Hydroxyurea-induced synchrony resulted in some distortion of the yeast-phase cell cycle. However, induction of synchrony by hydroxyurea is a rapid and simple technique which generates a marked degree of synchronous growth.