Expression of matrix metalloproteinases 2 and 9 in human trophoblasts of normal and preeclamptic placentas.

Abstract

Preeclampsia is a common and serious complication affects 3% to 7% of all pregnancies. MMPS is a family of proteolytic enzymes, able to degrade extracellular matrix & basement membrane components. Several studies have examined the activity and localization of MMP2 and 9 in vitro systems (tissue culture and amniotic fluid). To date, studies that bear directly on the tissue localization and expression pattern of MMP2 & 9 (IHC) in human preeclampsia are limited. This is a case–control study including preeclamptic placentas (N=40) and placentas of normal pregnancies (control group, N= 40). Through an immune-histo-chemical study, expression of MMP2 and 9 was examined using monoclonal antibodies against MMP 2 and 9. The negative control sections were treated in an identical manner with the omission of primary antibody. Positive control for MMP-2 was human placenta villous tissue (from first trimester). Positive control for MMP-9 was endothelial cells. Data were analyzed using SPSS for windows.