Comparative efficacy of seven selective media for isolating Campylobacter jejuni

Abstract

Diarrheal stools from 263 patients were inoculated on seven selective media: Butzler selective medium, Blaser medium, Skirrow blood agar, Preston campylobacter selective medium, Preston campylobacter blood-free medium, Butzler Virion medium, and modified Preston medium (with amphotericin B [2 mg/liter]). A similar number of Campylobacter jejuni strains were isolated from all the media studied; nevertheless, the presence of competing fecal flora (FF) made the detection of suspect colonies difficult. Preston campylobacter blood-free medium with cefoperazone yielded the greatest number of C. jejuni isolations, and contaminating FF grew in only 9% of the plates showing C. jejuni growth; all the other media allowed the abundant growth of other FF, regardless of whether C. jejuni was isolated from them or not.