Surface Plasmon Resonance-Based Protein Bio-Sensing Using a Kretschmann Configured Double Prism Arrangement

Abstract

In this paper, we demonstrate bio-sensing response of bio-medically relevant proteins by employing a surface plasmon resonance (SPR)-based experimental setup that uses a set of double prisms in Kretschmann configuration. The SPR signal of nanoscale Ag-films of varying thicknesses was first monitored experimentally and then verified through computational simulation. The SPR signal was found to be substantially modulated at the metal-dielectric interface when the nanoscale Ag-films are exposed to bio-molecules, such as catalase, papain, bovine serum albumin, trypsin, and lysozyme, which were evaluated independently.