Vox Sanguinis · 1973 · 49 citations · 8 references
Life SciencesBiochemistryClinical BiochemistryGood Laboratory PracticeHemostasisChromatographyPreparative SeparationProtein SeparationViral HepatitisPlasma Protein AdsorptionProtein Purification By AdsorptionProthrombin ActivationProthrombin Complex Concentrate UseTemperature Effects On Separation
Abstract. Two methods are described for the preparation of human prothrombin complex on a large scale. In the first method DEAE‐cellulose DE 52 is used as an adsorbent and in the second method DEAE‐Sephadex A‐50. The results obtained with both methods are compared. The DEAE‐cellulose method is an adaptation from a previously existing small‐scale to a large‐scale preparation. The DEAE‐Sephadex method is newly developed. The amount of DEAE‐Sephadex required for adsorption, the duration of adsorption and the influence of the temperature are examined. The behaviour of Hepatitis Associated Antigen (HAA) in both methods is tested and, it is argued, that by introducing an extensive washing the risk of transmitting serum hepatitis is considerably diminished. It is concluded that the DEAE‐Sephadex method is the most suitable for a routine large‐scale production of the prothrombin complex.
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