Polypeptide Synthesis in Extracts of Wheat Germ

Abstract

Abstract Polyphenylalanine synthesis in a wheat germ system requires GTP, polyuridylic acid, and two soluble aminoacyl transfer factors. Procedures are described for the complete resolution and partial purification of each of the aminoacyl transfer factors. One of the factors catalyzes a GTP and polyuridylic acid-dependent binding of phenylalanyl transfer RNA to ribosomes while the second factor facilitates the formation of peptidyl puromycin from nonenzymatically bound phenylalanyl-tRNA. Both of the factors catalyze a ribosome-dependent hydrolysis of GTP.