Antimicrobial potential of various extract and fractions of leaves of Solanum nigrum

Abstract

This study was carried out to evaluate the antimicrobial activity of methanolic extract and different fractions ( n -butanol, ethyl acetate, chloroform and n -hexane) of S.nigrum leaves. The antimicrobial activity was determined by the disc diffusion method and minimum inhibitory concentration (MIC) against a panel of microorganisms (four bacterial strains, i.e P. multocida, E. coli, B. subtilis and S. aureus and three fungal strains, i.e A . flavus, A. niger and R. solani) .The results indicated that leaf extract and fractions of S. nigrum were mildly potent as antibacterial agent. While Antifungal activity of S. nigrum leaves extract/fractions was poor . Keywords: Solanum nigrum, antimicrobial activity, Resazurin, A . flavus, Minimum inhibitory concentration