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A microsome-dependent binding of benzo[a]pyrene to DNA.
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1969
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Molecular PharmacologyNucleic Acid ChemistryUnknown MetaboliteBiochemistryMedicineNatural SciencesMolecular BiologyMicrosome-dependent BindingOrganic ChemistryToxicologyExperimental ToxicologyChemical BiologyPharmacologyPolycyclic Aromatic HydrocarbonSummary BenzoToxicological MechanismCalf Thymus DnaCarbonyl Metabolism
Summary Benzo[a]pyrene- 3 H incubated in the presence of rat liver microsomes with calf thymus DNA binds covalently to the DNA. The reaction requires reduced nicotinamide adenine dinucleotide phosphate and is greater when the microsomes are obtained from rats pretreated with polycyclic hydrocarbons. The binding is due to the formation of unknown metabolite(s) which then may bind to the DNA in the absence of microsomes.