Nucleotide sequence of the gene encoding pilin of Bacteroides nodosus, the causal organism of ovine footrot

Abstract

The nucleotide sequence encoding pilin, the monomer protein subunit of the pilus from Bacteroides nodosus, has been determined. The sequence predicts a short, positively charged, amino-terminal segment which is absent from the amino acid sequence of mature pilin. The coding sequence is preceded upstream by a sequence of five nucleotides complementary to the 3' end of 16S rRNA of Escherichia coli--a potentially good ribosome binding site--and even further upstream by an AT-rich region preceding several potential recognition sites for RNA polymerase. The coding sequence is followed by a region of hyphenated dyad symmetry having the potential to act as a rho-independent terminator of transcription.