Production and characterization of the novel CMS line of radish (<i>Raphanus sativus</i>) carrying <i>Brassica maurorum</i> cytoplasm

Abstract

With 1 figure and 1 table Abstract The alloplasmic line of radish carrying Brassica maurorum cytoplasm was obtained from four backcrosses (BC) of the amphidiploid hybrid ( B. maurorum × Raphanus sativus ) to radish. Eight BC 1 plants (2n = 26) and one BC 2 plant (2n = 22) were generated only by using embryo rescue, and two BC 3 plants (2n = 19, 20) were obtained by repeat pollinations after unbagged bud pollination and without embryo rescue. After successive backcrossings with 10 radish cultivars to BC 6 generation, the resulting alloplasmic line of radish (2n = 18) showed stable expression of male sterility without any recognizable changes in vegetative growth and female fertility. This cytoplasmic male sterility (CMS) line of radish was dissimilar to the other existing CMS lines with respect to cytogenetic and molecular characteristics. The novel CMS line produced in the present investigation may be a versatile germplasm for F 1 hybrid seed production of radish.