Studies on the site in the regulatory subunit of type I cAMP-dependent protein kinase phosphorylated by cGMP-dependent protein kinase.

Abstract

The amino acid sequence around the site of the regulatory subunit of type I cAMP-dependent protein kinase (RI) that is phosphorylated by cGMP-dependent protein kinase has been determined. This site was found to be located near the site on RI previously shown to be very sensitive to hydrolysis by trypsin (Potter, R. L., and Taylor, S. S. (1979) J. Biol. Chem. 254, 2413-2418). The primary sequence surrounding the site is as follows: -Lys-Ala-Gly-Ser-Arg-Ala-Asp-Ser-Arg-Glu-Asp-Glu-Ile-Ser-Pro-Pro-Pro-Pro-Asn-Pro-Val-Val-Lys-Gly-Arg-Arg-Arg-Arg-Gly-Ala-Ile-Ser(P)-Ala-Glu-Val-Tyr-Thr-Glu-Glu-Asp-Ala-Ala-Ser-Tyr-Val-Arg-Lys-Val-Ile-Pro-Lys-Asp-Tyr-Lys-Thr-. As described previously (Geahlen, R. L., and Krebs, E. G. (1980) J. Biol. Chem. 255, 1164-1169), this site is specific for cGMP-dependent protein kinase and is not phosphorylated by the catalytic subunit of cAMP-dependent protein kinase.