Synthesis of a key fluorogenic amide, L-arginine-4-methylcoumaryl-7-amide (L-Arg-MCA) and its derivatives. Fluorescence assays for trypsin and papain.

Abstract

L-Arginine-4-methylcoumaryl-7-amide 2 and its derivatives, which contain 7-amino-4-methylcoumarin 1 as a key fluorescence amine, were synthesized. The L-antipodes of Nα-benzoyl and Nα-carbobenzoxy derivatives were shown to be the useful fluorogenic substrates for the sensitive determinations of trypsin and papain.