Reaginic Antibody Receptors on Murine Mastocytoma Cells

Abstract

Abstract Sensitization for the allergic reaction requires the firm binding of IgE antibody in homologous tissues. Studies with monkey tissues (1) and with human leukocytes (2) show that IgE binds exclusively to mast cells and basophils. Electron micrographs show that IgE is bound to the plasma membrane of the human basophil (3). This localization of IgE suggests that these cells possess a specific membrane receptor for IgE. A mast cell tumor arose spontaneously in an LAF1 mouse which had been subjected to whole body radiation (4). This mastocytoma was originally a solid tumor and now also grows in an ascitic form. Receptors for homologous immunoglobulins on malignant mouse mast cells have recently been demonstrated by the technique of rosette formation with antibody-coated sheep erythrocytes (5–7). This report presents evidence for the ability of mouse mastocytoma cells to deplete serum of skin sensitizing activity, and suggests that these cells may have receptors for homologous reaginic antibody.