Cell Division of <i>Escherichia coli</i> BUG-6: Effect of Varying the Length of Growth at the Nonpermissive Temperature

Abstract

When Escherichia coli BUG-6 is grown at 42 C and then returned to 30 C, the division kinetics during the recovery at 30 C are dependent on the length of time the cells were grown at 42 C. If chloramphenicol is added when the cells are shifted from 42 to 30 C, no division occurs if the period at 42 C is less than 4 min or more than 110 min. Maximum division occurs when the period at 42 C is 50 min. A discussion of these results with reference to a previously proposed model is presented.