Productioin of Tetraploid Forms of Acid Citrus Cultivars by Top Grafting of Shoots with Sprouting Axially Buds Treated with Colchicine

Abstract

To induce the tetraploid forms of three diploid acid Citrus cultivars (2n=2x= 18), 'Yuzu' (C. junos), 'Kizu'(C. kizu) and 'Hanayu' (C. hanaju), sections of one-year-old spring shoots with sprouting axillary buds were treated with various concentrations of colchicine and immediately top=grafted on 30-year-old satsuma mandarin trees at the beginning of April.