Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

Abstract

Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a continuous-sample-flow-with-isopycnic-banding centrifuge in quantities up to 6 x 10(13) polyhedral inclusion bodies per day. Continuous-flow methods for S-rho type purification have been evolved to deal with mass isolation of bioparticles.