Cloning and location of the dgsA gene of Escherichia coli

Abstract

The dgsA locus of Escherichia coli was isolated on plasmids obtained from the library of L. Clarke and J. Carbon (Cell 9:91-99, 1976). Restriction fragment analysis and further subcloning demonstrated that the gene is located at kilobase 425 on the Bouché physical map of the terminus region (J. P. Bouché, J. Mol. Biol., 154:1-20, 1982). This corresponds to 35.2 min on the Bachmann genetic map (B. J. Bachmann, Microbiol. Rev. 47:180-230, 1983).