UPTAKE OF VALINE AND GLYCYLVALINE BY <i>LEUCONOSTOC MESENTEROIDES</i>

Abstract

Shelton, Damon C. (West Virginia University Medical Center, Morgantown), and William E. Nutter. Uptake of valine and glycylvaline by Leuconostoc mesenteroides. J. Bacteriol. 88:1175-1184. 1964.-Uptake systems for valine and glycylvaline were studied in growing cultures and non-growing cellular suspensions of Leuconostoc mesenteroides. Glycylvaline promoted growth more rapidly than did valine. Other dipeptides were shown to be antagonistic to the utilization of glycylvaline by the organism. The experiments with cellular suspensions were done with the use of combinations of labeled and unlabeled amino acids and peptides. Results showed that different uptake systems were present in L. mesenteroides for the uptake of valine and glycylvaline. Both systems were energy-dependent and readily saturated. The synthesis, purification, and chromatographic identification of glycyl-dl-valine-1-C(14) is described.