Analysis of Amino Acids by High-Performance Liquid Chromatography with Circular Dichroism Detection.

Abstract

Stereochemical detection and separation of the enantiomers of DL-amino acids were performed using a high-performance liquid chromatograph (HPLC) equipped with a chiral column and a circular dichroism spectrophotometer as a detector. Various racemic amino acids were separated by the chiral column and all L-amino acids and D-amino acids were detected as positive and negative circular dichroism peaks, respectively. Mixtures of two amino acids, DL-norleucine and DL-norvaline, DL-phenylglycine and DL-dopa, and glycine and DL-leucine were also analyzed.