Fine mapping and identification of the soybean <i>R</i>_<i>SC4</i> resistance candidate gene to soybean mosaic virus

Abstract

With 3 figures and 3 tables Abstract Soybean mosaic virus (SMV) disease is one of the most destructive viral diseases for soybeans worldwide. In this study, 1047 F 2 plants derived from ‘Dabaima’ (resistant) × ‘Nannong1138‐2’ (susceptible) were used to study inheritance and resistance gene linkage to SMV strain SC4. SSR were used to establish genetic maps of the parent plants, in addition to resistant or susceptible F 2 generation plants. Results indicated that a single dominant gene (designated R SC4 ) located on chromosome 14 (MLG B2) controls resistance to SC4 infections. In addition, SNP and genomic‐SSR markers near R SC4 were examined. Based on the linkage analysis of the population, the genomic‐SSR markers BARCSOYSSR_14_1413 and BARCSOYSSR_14_1416 were found flanking R SC4 . Sequence analysis of the soybean genome indicated that the interval between the two genomic‐SSR markers was &lt;100 kb on chromosome 14. Quantitative real‐time PCR (QRT‐PCR) analysis of the 100‐kb region identified three genes (Glyma14g38510, 38560 and 38580) likely involved in regulating resistance to SMV. These results are useful for transferring and pyramiding or map‐based cloning of R SC4 .