Enrichment Medium for Selection of <i>Salmonella</i> from Fish Homogenate

Abstract

A new liquid medium, called "dulcitol selenite enrichment," has been developed for the detection and enumeration of Salmonella in foods. The medium is not only highly selective in enriching Salmonella and inhibiting completely or appreciably other extraneous organisms commonly found in seafoods, but is also highly sensitive in recovering as low as 2 to 7 cells of Salmonella, even in the presence of large numbers (10(4) to 10(6) cells) of mixed flora common to these foods. The addition of seafood material does not seem to interfere with the sensitivity, selectivity, or productivity of the medium. Even physiologically debilitated cells of Salmonella were enriched well enough in this medium to be detected easily.