Development of a cDNA‐AFLP derived CAPS marker co‐segregating with the powdery mildew resistance gene <i>Mlg</i> in barley

Abstract

Abstract Powdery mildew is an important pathogen of barley in many barley‐growing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA‐AFLP approach was carried out on near‐isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by blastn , a CAPS marker has been developed, which co‐segregated with Mlg in a population of 738 F 2 ‐plants. Due to its co‐dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker‐based selection procedures.