Fusarium semitectum mediated extracellular synthesis of silver nanoparticles and their antibacterial activity

Abstract

Method Collection of MaterialsFusarium semitectum was isolated from soil and maintained on potato dextrose agar (PDA) medium at 28C.The isolated fungus was identified using morphological characterization.The clinically isolated two kinds of bacteria Bacillus subtilis and Staphylococcus sp. were tested for their susceptibility against silver nanoparticles. Biomass PreparationFusarium semitectum was grown in Glucose nutrient broth medium (GNB) for biomass preparation.The flask was inoculated with spores and incubated at 28C on a rotatory shaker (120 rpm) for 4 days.The biomass was harvested by filtration through filter paper (Whatman filter paper no-1) and then washed with distilled water to remove any components of the medium.10 gm biomass was placed in individual flasks containing 100 ml double-distilled water.The flask was incubated for 72 hr.The biomass was again filtered by Whatman filter paper no-1 and the crude cell free filtrate was collected for experiment. 2.3Biosynthesis of Silver NanoparticlesSilver nanoparticles were synthesized using 10 ml cell free filtrate mixed with 10 ml of 1 mM AgNo 3 solution in 250 ml of Erlenmeyer flask was incubated at 28C in dark for 24 hr.AgNo3 solution was used as control.