Studies with Specific Enzyme Inhibitors

Abstract

Isolation of D and L isomers of erythro-fluorocitric acid was accomplished by first separation of diastereomeric racemates as cyclohexylamine salts of diethyl fluorocitrate.This was followed by resolution of the erythro-diastereoisomer into optical isomers by fractional crystallization as (+)-and ( -)deoxyephedrine salts.Partial hydrolysis to the monoethyl ester of resolved fluorocitrate was achieved by guanidine carbonate and complete hydrolysis to the free acid in Na0I-I.Puriiication to the &ml stage of optically resolved free acids was carried out by fractionation as barium and cyclohexylamine salts.Only the L isomer inhibited aconitase activity of rat kidney mitochondria.