INCORPORATION IN VITRO OF LABELED AMINO ACIDS INTO RAT DIAPHRAGM PROTEINS

Abstract

We have reported the incorporation of labeled amino acids into the proteins of rabbit bone marrow cells in vitro (1), a study that was undertaken to compare the process in intact cells as compared with tissue slices and homogenates. Bone marrow cells are a mixture in different stages of maturity. The uptake of labeled amino acids by rat diaphragm was studied because it is an adult tissue with predominantly one type of cell and it can be removed from the animal with little damage. This preparation has been found useful in studies of carbohydrate metabolism of muscle in vitro (2).