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Establishment of a cell line (Co-115) from a human colon carcinoma transplanted into nude mice.
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1976
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PathologyCancer BiologyCulture MediumTumor BiologyGastrointestinal OncologyCancer Cell BiologyCell TransplantationCancer ResearchSolid XenograftsMedicineColorectal CancerCell LineMalignant DiseaseSolid Tumor MassesCell BiologyTumor MicroenvironmentNude MiceHuman Colon CarcinomaOncology
A human colon carcinoma cell line, Co-115, has been established in vitro from solid xenografts maintained in nude mice and subcultured for 95 passages. Co-115 cells grow in vitro as tightly packed, epithelial-like colonies, have a doubling time of about 36 hr, have a relatively low plating efficiency in agar, and release significant amounts of carcinoembryonic antigen to the culture medium. Their epithelial nature has been confirmed by ultrastructural examination. The injection of Co-115 cells into nude mice reinduced the formation of solid tumor masses that could be retransplanted and showed a morphology comparable of that of the original xenograft.