A new, simple, nonradioactive, nontoxic in vitro assay to monitor corneal endothelial cell viability.

Abstract

Alamar blue reduction measures endothelial cell viability and can readily differentiate cell concentrations. It demonstrates several advantages over [3H]-thymidine: It can assay nonproliferating endothelial cell metabolism, it allows rapid assessment of large numbers of samples, it can differentiate endothelial cell concentrations, it is nontoxic, it is nonradioactive and allows for simple disposal, it is less costly, and it allows for continuous monitoring of endothelial cell metabolism and viability.