Studies on the cultivation of poliomyelitis viruses in tissue culture. II. The propagation of the poliomyelitis viruses in roller-tube cultures of various human tissues.

Abstract

The procedures employed for the propagation of these agents in human tissues have been described in detail and the effect of certain modifications in technique on the growth of the virus has been determined. The advantages and disadvantages of the roller culture as compared with the suspended cell culture for the propagation of poliomyelitis viruses have been discussed and it is concluded that for most purposes the former is to be preferred.