Method to Introduce Stable, Expanded, Polyglutamine-Encoding CAG/CAA Trinucleotide Repeats into CAG Repeat-Containing Genes

Abstract

Fiber DNA measurements were performed using two different microscope settings (Leica and Olympus) in two different laboratories with identical results. Our data indicate that this fast and simple procedure allows DNA size measurements that are as accurate or more accurate (5%-10%) than those taken by gel electrophoresis.