Journal of Biological Chemistry · 1984 · 143 citations · 16 references
Life SciencesBiochemistryMolecular BiologyGenetic EngineeringMicrobial GeneticsRecombinant Dna TechnologyMolecular CloningPlasmid BiologyPlasmid EngineeringPlasmid GeneticsRecombinant Plasmid ConstructionRecombinant Protein ProductionBacterial Plasmid ConstructionEscherichia ColiPlasmid Dna PurificationPlasmid Gene TransferPlasmid IsolationPlasmid TransferPlasmid Transfer SystemsPurification
We have transferred the Escherichia coli gyrA and gyrB genes onto plasmids that allow the overproduction of the DNA gyrase A and B proteins and have designed relatively simple purification procedures for both proteins. The pure proteins are obtained in good yield; from 2 liters of culture (12 g of cells), one can recover 25 mg of GyrA or 3 mg of GyrB protein.
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Akio Sugino, Craig L. Peebles, Kenneth N. Kreuzer et al. · Proceedings of the National Academy of Sciences · 1977 · 782 citations · Full text