Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype.

Abstract

Summary Two types of clonal strains have been derived from adrenal tumor cultures. One type is morphologically fibroblastic and does not perform adrenal-specific functions. The 2nd type is epithelial in morphology and is extensively similar in properties to normal adrenal cortex. Epithelial clones have maintained their functional characteristics for over a year in continuous culture. They can undergo reversible variations in functional activities in culture. The epithelial clones are near diploid with a stem line of 39 chromosomes.