Biologic activity of soluble antigen-antibody complexes. V. Change of optical rotation by the formation of skin reactive complexes.

Abstract

Summary 1.The optical activity of soluble antigen-antibody complexes was studied. 2.It was found that levorotation increases in the formation of skin reactive BSA-anti-BSA complexes, whereax it does not change on the formation of the Ag 2 Ab complex which is inactive in the skin reaction. 3.The increase of levorotation was observed by the formation of skin reactive soluble complexes composed of synthetic precipitating R 3 9-dye and homologous antibody, whereas it does nto change on the formation of simple hapten (R)-anti-R complexes. 4.These results support our hypothesis that the toxicity of soluble complexes is due to molecular changes brought about in the antibody molecules.