Increase of leukemia L1210 immunogenicity by Vibrio cholerae neuraminidase treatment.

Abstract

Modification of leukemia L1210 tumor cells with Vibrio cholerae neuraminidase resulted in loss of oncogenicity; 107 treated cells failed to induce leukemia in DBA/2 mice. Immunogenicity of the treated tumor cells was increased, since DBA/2 mice were specifically resistant to 105 virulent L1210 cells after single immunization. Immunogenicity is directly correlated with release of neuraminidase-susceptible N -acetylneuraminic acid. However, prolonged incubation with neuraminidase, beyond 90 min for this particular tumor, gradually destroys the immunogenicity of the tumor cells.