DEVELOPMENT OF AN HPLC METHOD FOR THE DETERMINATION OF GLYCEROL OXIDATION PRODUCTS

Abstract

An HPLC method for the separation of glycerol oxidation products, namely glyceraldehyde, dihydroxyacetone, mesoxalic, tartronic, and glycolic and glyceric acids on an ion-exchange 8% cross-linked calcium sulfonated divinylbenzene-styrene resin column was developed and validated. The conditions reported include temperature (70°C), flow rate (0.5 mL/min) and concentration of the mobile phase (3 mM H2SO4) using isocratic elution with ultraviolet and refractometric detectors. The effect of the mobile phase flow rate and concentration as well as column temperature on the resolution of peaks is described. Excellent correlation coefficient in the calibration model was observed for all analytes over the concentration range of 0.5 to 10 mg/mL. The method was also validated in terms of intra-day precision, sensitivity, accuracy, and detection and quantification limits. The method conditions were applied to the identification of products derived from the chemical oxidation of glycerol. Supplemental materials are available for this article. Go to the publisher's online edition of the Journal of Liquid Chromatography & Related Technologies to view the supplemental file.