Determination of fluorescein and fluorescein monoglucuronide excreted in urine.

Abstract

A simple method for the fluorometric determination of fluorescein and fluorescein monoglucuronide in urine was developed. Free fluorescein was determined by measuring its native fluorescence at 494 nm excitation and 516 nm emission. The conjugate was determined as fluorescein after hydrolysis at 37°for 20 min with β-glucuronidase. This method was applied to investigate the glucuronidation function of Wistar rats with liver injury in comparison with that of normal rats.