Sensitivity of the response of cytosolic calcium in Quin-2-loaded rat hepatocytes to glucagon, adenine nucleosides, and adenine nucleotides.

Abstract

Hepatocytes from fasted rats were used to study the effect of glucagon on intracellular free cytosolic Ca2+ ([Ca2+]i) indicated by the use of Quin-2-calcium fluorescence. It was found that, in both male and female rats, glucagon increased [Ca2+]i at a half-maximally effective concentration (Kact) of 0.3 nM, a concentration known to be half-maximal for affecting several hepatic functions. Acute chelation of extracellular Ca2+ did not obliterate the hormone effect but shortened its duration. Cyclic AMP, 5'-AMP, ADP, and ATP also increased [Ca2+]i, while adenosine 2':3'-monophosphate and 3'-AMP did not. The rise in [Ca2+]i brought about by glucagon at near physiological concentrations may be responsible for the stimulation of glutamate metabolism produced acutely by glucagon.