Ion Transport and Respiratory Control in Vesicles Formed from Cytochrome Oxidase and Phospholipids

Abstract

Abstract Vesicles formed from cytochrome oxidase and phospholipids catalyzed the oxidation of reduced cytochrome c in a manner similar to that of intact mitochondria. A respiration-dependent release of protons and uptake of potassium ions were observed in the presence of valinomycin with reduced 1,4-naphthoquinone-2-sulfonate as reductant for cytochrome c. With ascorbate as reductant 3- to 5-fold stimulation of respiration was observed on addition of uncouplers or ionophorous agents.