Journal of Biological Chemistry · 1972 · 256 citations · 8 references
Life SciencesBiochemistryCell BiologyBioenergeticsMitochondrial BiologyRedox BiochemistryCellular RespirationMitochondrial BioenergeticsMitochondrial MembranesMitochondrial MetabolismMitochondrial PhysiologyOxidative PhosphorylationCytochrome OxidasesIon TransportMitochondrial Respiration ControlMitochondrial Respiration InhibitionMitochondrial Respiratory ComplexesOxidase CatalysisRespiratory Rate Control
Abstract Vesicles formed from cytochrome oxidase and phospholipids catalyzed the oxidation of reduced cytochrome c in a manner similar to that of intact mitochondria. A respiration-dependent release of protons and uptake of potassium ions were observed in the presence of valinomycin with reduced 1,4-naphthoquinone-2-sulfonate as reductant for cytochrome c. With ascorbate as reductant 3- to 5-fold stimulation of respiration was observed on addition of uncouplers or ionophorous agents.
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