Development of a real‐time polymerase chain reaction assay for sensitive detection and quantitation of <scp><i>Babesia microti</i></scp> infection

Abstract

We have developed a highly sensitive and specific, quantitative real-time PCR-based assay for detection of B. microti that could have a useful role in blood screening. It can also be employed broadly to understand Babesia epidemiology, disease pathogenesis, and host immunology.