Journal of Bacteriology · 1992 · 36 citations · 25 references
Life SciencesMicrobiologyMolecular BiologyGene ExpressionMicrobial GeneticsTranscriptionBacterial Gene RegulationPromoter ArchitecturePromoter RecognitionTranscription In ProkaryotesTranscription Start SitesTranscriptional Regulation In ProkaryotesAmino AcidsBacterial PromotersCap Binding ProteinsDna Binding SpecificityHelix RecognitionPositionPromoter SpecificityTranscription In Bacteria
The Xanthomonas campestris catabolite gene activator protein-like protein (CLP) can substitute for the Escherichia coli catabolite gene activator protein (CAP) in transcription activation at the lac promoter (V. de Crecy-Lagard, P. Glaser, P. Lejeune, O. Sismeiro, C. Barber, M. Daniels, and A. Danchin, J. Bacteriol. 172:5877-5883, 1990). We show that CLP has the same DNA binding specificity as CAP at positions 5, 6, and 7 of the DNA half site. In addition, we show that the amino acids at positions 1 and 2 of the recognition helix of CLP are identical to the amino acids at positions 1 and 2 of the recognition helix of CAP:i.e., Arg at position 1 and Glu at position 2.
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