Peripheral synthesis and isoform distribution of dog apoprotein E. An in vivo approach.

Abstract

Purified dog plasma apo-E is composed of four major isoforms with the following PI values: 5.40,5.31,5.26,and 5.22.Treatment with neuraminidase suggests that the multiple forms are due to progressive sialation.The acidic isoforms (PI = 5.22 and less), rarely detectable in plasma lipoprotein samples (except in the d c 1.05 g/ml fraction of cholesterol-fed dogs), are present in high concentrations in the interstitial fluid high density lipoprotein fraction I (HDLI) of cholesterol-fed dogs, a lipoprotein recently described (Dory, L., Bo-