Age-related changes in the structure of the proteoglycan subunits from human articular cartilage.

Abstract

Materials-Guanidinium chloride, cesium chloride, and toluidine blue were obtained from BDH Chemicals, Ltd.Phenylmethylsulfonyl fluoride, iodoacetic acid, papain, bacterial collagenase, and hyaluronic acid (from human umbilical cord) were from Sigma Chemical Co.Pepsin was from Worthington Biochemical Cow. Chondroitinase ABC and its disaccharide degradation products of chondroitin sulfate were from Miles Laboratories, Inc. Acrylamide, N,N'-methylenebisacrylamide, and Coomassie